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mip waterfall image

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mip waterfall image

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What makes MIPs so unique?:

Molecular imprinting is a technique for introducing selectivity towards a specific compound, or a class of compounds, during the creation of the sorbent material. This is achieved by using a template molecule chosen to be representative of the 3-dimensional shape and functional chemistries of the target compound, or class, of interest. Functional monomers are added, which form complexes with the template molecule. A polymer is then formed to include the functional monomers, creating a mould around the template. Once the template molecule is removed, the polymer is left with imprints of the molecule (binding sites) that complement the 3-D shape and functional groups of its intended target molecules. See schematic Making a MIP.

The MIP is comparable to a man-made antibody or receptor in terms of selectivity, but has the stability of a polymer material. Because MIPs are highly cross-linked polymers, they are very stable, even when used with extreme pHs, a wide variety of organic solvents and various temperatures.

Cleaner Extracts; Lower Detection Limits:

Due to the specificity engineered into MIP production, MIPs exhibit stronger binding affinity toward their target analyte(s) than to sample matrix components. Consequently, matrix components and other undesirables can be washed away during the sample extraction process, leading to:

  • Cleaner extracts, reduced background interference

  • Lower chemical noise

  • Lower detection limits/improved sensitivity

  • Consistently high target analyte recovery

  • Elimination of ion-suppression in LC/MS applications

Simple and Cost-Effective:

Since sample preparation is often a time-consuming & rate limiting step, MIPs also provide significant economic benefits by enabling:

  • Reduced sample volumes

  • Fewer sample clean-up steps

  • Reduced solvent consumption

  • Shorter sample preparation time

  • Lower labour cost


Making
a MIP:

molecularly imprinted polymer template
Template Molecule

+

molecularly imprinted polymer functional monomers
Functional Monomers

arrow

molecularly imprinted polymer template complex
Template Complex

+


molecularly imprinted polymer cross-linker monomer
Cross-linker Monomer

 

arrow
Polymerization

molecularly imprinted polymer formation

arrow
Template Removal

molecularly imprinted polymer empty
target analyte

equilibrium arrows

molecularly imprinted polymer at work

Selective Molecularly Imprinted Polymer (MIP)

 

 

In short, MIPs deliver better results, greater productivity, and lower costs.


See specific MIP[4]SPE products available:

  • Amphetamines (class specific)

  • beta-Agonist drugs (class specific)

  • beta-Blocker drugs (class specific)

  • beta-Receptor (beta-Agonists + beta-Blockers)

  • Chloramphenicol

  • Clenbuterol

 


Example:

  • Fluoroquinolones (class specific)

  • Nitroimidazoles (class specific)

  • NNAL (carcinogenic tobacco-specific nitrosamine)

  • Non-steroidal anti-inflammatory drugs (NSAIDs)

 
molecularly imprinted polymer chromatogram
  • Polycyclic Aromatic Hydrocarbons (PAHs)

  • Riboflavin (Vitamin B2)

  • Tobacco-specific nitrosamines (NNN, NNK, NAB, & NAT)

  • Triazine herbicides (class specific)

  • ExploraSep Method Development Kits

  • others are in development

 

 

The chromatograms show the extraction of a typical analyte from a 5 mL urine sample. Superior clean-up with the MIP sorbent is shown in red, whereas the other chromatograms show clean-up with a much higher level of chemical noise, using mixed-phase SPE sorbents.


More information is available:

An extensive collection of literature is available, including:

  • Product Bulletins for specific target analytes, or classes

  • Application Notes for specific matrices

  • Published Papers

  • Poster Presentations

See our complete list to request more information.

 
       
         

 


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